| Gene Information | |
| NCBI Gene ID | 836723 |
| Symbol | ZWI |
| LocusTag | AT5G65930 |
| Synonyms | K14B20.10|K14B20_10|KCBP|KINESIN-LIKE CALMODULIN-BINDING PROTEIN|PKCBP|ZWICHEL |
| Description | ZWI (ZWICHEL); calmodulin binding / microtubule motor |
| Gene Type | protein-coding |
| Pattern | ZWI|AT5G65930|K14B20.10|K14B20_10|KCBP|KINESIN-LIKE CALMODULIN-BINDING PROTEIN|PKCBP|ZWICHEL|ZWI (ZWICHEL); calmodulin binding / microtubule motor |
| Corresponding sequence in Trichome EST library | AI780429 AJ848576 AM817479 AM827608 AW687922 BE249335 BE325480 BE999515 BI270215 BP528710 BP888391 BP906965 BP908480 BP910408 BQ141295 BQ164820 BQ165123 BQ255335 BQ750438 BQ750587 BT051144 BT051145 BT051146 BT053589 CA920688 CA921923 CX524424 CX527129 CX527548 DV105167 DV161078 DV161512 DY616780 EV257007 EY108708 EY115164 EY115165 EY477233 TCHL59938 TCMT50965 TCMT51008 TCMT54695 TCMT59900 TCMT60657 |
| Reference |
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Essential role of a kinesin-like protein in Arabidopsis trichome morphogenesis. Oppenheimer DG, Pollock MA, Vacik J, Szymanski DB, Ericson B, Feldmann K, Marks MD. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6261-6. ...... of trichomes (plant hairs) on the plant Arabidopsis thaliana as a model to study how cell shape is controlled. Wild-type Arabidopsis trichomes are large, single epidermal cells with a stalk and three or four branches, whereas in zwichel (zwi) mutants the trichomes have a shortened stalk and only two branches. To further understand the role of the ZWI gene in trichome morphogenesis we have cloned the wild-type ZWICHEL (ZWI) gene by T-DNA tagging, and report here that it ...... |
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Developmental and cell-specific expression of ZWICHEL is regulated by the intron and exon sequences of its gene. Reddy VS, Reddy AS. Plant Mol Biol. 2004 Jan;54(2):273-93. ...... Functional studies with ZWICHEL ( ZWI ), which encodes a Ca(2+)-calmodulin-regulated kinesin, have shown its involvement in trichome morphogenesis and cell division. To identify regulatory regions that control the ZWI expression pattern, we generated transgenic ...... |
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...... entire root including root hairs and root tip, calli and at various developmental stages in trichomes and pollen. However, very little GUS accumulation was detected in roots of dark-grown or root tips of cold-treated seedlings with 5' ZWI constructs. These results were further confirmed by quantifying GUS enzyme activity and transcripts in these seedlings. Calli and pollen transformed with the 5' distal 268 bp fused in antisense orientation to the proximal 460 bp did not ...... |
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...... GUS expression. Further, IAA-treated dark-grown seedlings with 726::GUS, but not with 460::GUS, showed high GUS expression in specific regions (outer layer 2a cells) at the base of the lateral roots. The ZWI 3' region (3 kb) did not influence the GUS expression pattern driven by the 5' 726 bp. The absence of CHY1 transcripts in the chy1-2 mutant did not alter either ZWI expression or ZWI-mediated trichome morphogenesis. Thus, our data suggest ...... |
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Microtubule stabilization leads to growth reorientation in Arabidopsis trichomes. Mathur J, Chua NH. Plant Cell. 2000 Apr;12(4):465-77. ...... drugs. The growth reorientation appeared to be linked to increased microtubule stabilization and to aster formation in the treated trichomes. Taxol-induced microtubule stabilization also led to the initiation of new branch points in the zwichel mutant of Arabidopsis, which is defective in a kinesin-like microtubule motor protein and possesses trichomes that are less branched. Our observations suggest that trichome cell branching in Arabidopsis might be mediated by transiently ...... |
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The actin cytoskeleton is required to elaborate and maintain spatial patterning during trichome cell morphogenesis in Arabidopsis thaliana. Mathur J, Spielhofer P, Kost B, Chua N. Development. 1999 Dec;126(24):5559-68. ...... trichome phenotype characterized by isotropic growth and a severe inhibition of branch formation; these trichomes did not show defects in actin cytoskeleton organization. A normal actin cytoskeleton was also observed in trichomes of the zwichel mutant which have reduced branching. ZWICHEL, which was previously shown to encode a kinesin-like protein is thought to be involved in microtubule-linked processes. Based on our results we propose that microtubules establish the spatial ...... |
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Extragenic suppressors of the arabidopsis zwi-3 mutation identify new genes that function in trichome branch formation and pollen tube growth. Krishnakumar S, Oppenheimer DG. Development. 1999 Jun;126(14):3079-88. ...... localized expansion events are initiated remain obscure. Mutational analysis of the trichome (plant hair) morphogenic pathway in Arabidopsis has identified at least eight genes that determine trichome branch number. One of these genes, ZWICHEL (ZWI), encodes a novel member of the kinesin superfamily of motor proteins. Mutations in the ZWI gene cause a reduction in the number of trichome branches. To identify additional genes involved in trichome branch initiation, we screened ...... |
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...... branch number defect of another branch mutant, furca1-2. Plants homozygous for suz2 have more than the wild-type number of trichome branches. This suggests that SUZ2 is a negative regulator of trichome branching and may interact with ZWI and FURCA1. The suz1 and suz3 mutants display no obvious phenotype in the absence of the zwi-3 mutation. The suz1 zwi-3 double mutants also exhibited a male-sterile phenotype due to a defect in pollen tube germination and growth, whereas ...... |
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Genetics of plant cell shape. Oppenheimer DG. Curr Opin Plant Biol. 1998 Dec;1(6):520-4. ...... are poorly understood. Genetic dissection of the trichome (plant hair) branching pathway in Arabidopsis, has uncovered mechanisms and identified genes that control plant cell morphogenesis. The recent identification of one of these genes, ZWICHEL (ZWI), as a novel member of the kinesin superfamily of microtubule motors provides a starting point for the analysis of the plant cytoskeleton's role in a specific morphogenetic event. ...... |