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| Title |
Cloning and promoter analysis of the cotton lipid transfer protein gene Ltp3(1). |
| Author |
Liu HC, Creech RG, Jenkins JN, Ma DP. |
| Issue |
Biochim Biophys Acta. 2000 Aug 24;1487(1):106-11. |
| Abstract |
A cotton Ltp3 gene and its 5' and 3' flanking regions have been cloned with a PCR-based genomic DNA walking method. The amplified 2.6 kb DNA fragment contains sequences corresponding to GH3 cDNA which has been shown to encode a lipid transfer protein (LTP3). The gene has an intron of 80 bp which is located in the region corresponding to the C-terminus of LTP3. The Ltp3 promoter was systematically analyzed in transgenic tobacco plants by employing the Escherichia coli beta-glucuronidase gene (GUS) as a reporter. The results of histochemical and fluorogenic GUS assays indicate that the 5' flanking region of the Ltp3 gene contains cis-elements conferring the trichome specific activity of Ltp3 promoter.
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| Link |
11004611 |
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