Location:
EST Analysis
> EST Library Details
| Library Acc. |
HL_TRI |
| Name |
Humulus lupulus (L. cultivar Phoenix) Trichome isolated from the female flower at mid-developmental stage |
| Organism |
Humulus lupulus |
| Tissue |
Trichome isolated from the female flower |
| Stage |
female flower at mid-developmental stage |
| Description |
Vector: pDNR-LIB; Site_1: Sfi IA (GGCCATTACGGCC); Site_2: Sfi IB (GGCCGCCTCGGCC); The mid-developmental stage female flower (cone) of Hops (Humulus lupulus) plants, cultivar Phoenix, were collected form plants grown at the Hop Research Institite, Wye, Kent, UK. The cones were stripped off the branches, and the largest cones (which were unlikely to develop much further), and the smaller cones (which contain few trichomes) were discarded. Total RNA isolated from hops trichome prepared by shaking the cones in a 50 mL tubes by using Cold-Phenol protocol. The cDNA library from hops trichome was constructed using the Creator Smart cDNA library construction kit (Clontech, Palo Alto, CA, USA) following the manufacturer's protocol for total RNA. |
| # of EST/cDNA |
12360 |
| # of Unigene |
7185;
Of them, 5471 singletons and 1714 TCs.
|
| Download (.zip) |
link |
| Release Date |
Oct 09, 2007 |
| Contact |
Zhao Xuechun Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73402, USA Tel: 580 224 6725 Fax: 580 224 6692 Email: pzhao@noble.org. |
| Citation |
Analysis of cDNA libraries from trichome of Hops (Humulus lupulus)
Wang,G., Aziz,N., King,A., Zhao,X. and Dixon,R.A.
Unpublished (2007)
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