Location:
EST Analysis
> EST Library Details
| Library Acc. |
MTUS_MIXTISSUE |
| Name |
Medicago truncatula mixed tissues |
| Organism |
Medicago truncatula |
| Tissue |
mixed tissues |
| Stage |
various stages |
| Description |
Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells. |
| # of EST/cDNA |
6743 |
| # of Unigene |
4979;
Of them, 433 singletons and 4546 TCs.
|
| Download (.zip) |
link |
| Release Date |
Jul 21, 2003 |
| Contact |
VandenBosch K Department of Plant Biology University of Minnesota 220 BioSci Center, 1445 Gortner Ave, St. Paul, MN 55108, USA Tel: 612 624 2755 Fax: 612 625 1738 Email: kvandenb@cbs.umn.edu TIGR sequence name: MTUSZ96TK Alias Clone name:pGVSN-15M9 More information is available at: www.medicago.org Seq primer: SKmod (CTA gAA CTA gtg gAT CC). |
| Citation |
The Medicago truncatula 6K unigene set: cDNA clones selected and re-arrayed from various libraries
VandenBosch,K., Endre,G., Silverstein,K, Town,C.D., Van Aken,S., Utterback,T., Cheung,F. and Fraser,C.M.
Unpublished (2002)
|
|
