Location:
EST Analysis
> EST Library Details
| Library Acc. |
MtBC_GLOMUS |
| Name |
Medicago truncatula, noculated with Glomus intraradices |
| Organism |
Medicago truncatula |
| Tissue |
arbuscular mycorrhiza |
| Stage |
harvested 3 weeks post inoculation with Glomus intraradices |
| Description |
Vector: pBluescript pSK; Site_1: EcoRI; Site_2: XhoI; M. truncatula sterilised seeds were germinated for 72h at 25 C, before transplanting into a 1/3 Epoisses soil : 2/3 calcined Terragreen mix in the presence of onion root fragments colonized by the arbuscular mycorrhizal fungus Glomus intraradices (Schenck & Smith, isolate LPA8). The plants were watered every day and twice a week with a modified nutrient Long Ashton solution without phosphate but with a high level of nitrate. After 3 weeks RNA was extracted from whole root systems. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into Uni-zap XR vector from Stratagene and packaged using Gigapack Gold packaging extracts. Plasmids containing cDNA inserts were mass-excised from phage stocks using ExAssit helper phage and propagated in SOLR cells. Clone ordering and sequencing was performed by the Centre National de Sequencage (Genoscope, Evry, France). Note : EST may be of fungal origin. |
| # of EST/cDNA |
8596 |
| # of Unigene |
4000;
Of them, 1026 singletons and 2974 TCs.
|
| Download (.zip) |
link |
| Release Date |
Aug 03, 2000 |
| Contact |
Genoscope Genoscope - Centre National de Sequencage BP 191 91006 EVRY cedex - FRANCE Email: seqref@genoscope.cns.fr, Web : www.genoscope.cns.fr Contact : Pascal Gamas and Etienne-Pascal Journet, Laboratoire de Biologie Moleculaire des Relations Plantes-Microorganismes, CNRS-INRA, BP 27 31326 Castanet-Tolosan Cedex, France (Email : Mt-est@toulouse.inra.fr Website : http://sequence.toulouse.inra.fr/Mtruncatula.html). |
| Citation |
Medicago truncatula ESTs from endomycorrhizal roots
Journet,E.P., Crespeau,H., van-Tuinen,D., Gouzy,J., Jaillon,O., Niebel,A., Carreau,V., Chatagnier,O., Kahn,D., Gianinazzi-Pearson,V. and Gamas,P.
Unpublished (2000)
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