Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_GSEED |
| Name |
Medicago truncatula Germinating Seed |
| Organism |
Medicago truncatula |
| Tissue |
germinating seeds |
| Stage |
0, 1, 2 and 3 days after acid treatment. |
| Description |
Vector: Lambda Zap; M. truncatula seeds were acid treated and placed on wet filter papers in petri dishes. Seeds were harvested at 0, 1, 2 and 3 days after acid treatment. cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each time point. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells. |
| # of EST/cDNA |
7440 |
| # of Unigene |
3909;
Of them, 863 singletons and 3046 TCs.
|
| Download (.zip) |
link |
| Release Date |
Jan 12, 2005 |
| Contact |
May GD Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73402, USA Tel: 580 224 6650 Fax: 580 224 6692 Email: gdmay@noble.org. |
| Citation |
Medicago truncatula Germinating Seed Expressed Sequence Tags from the Samuel Roberts Noble Foundation - Center for Medicago Genomics Research
Torres-Jerez,I., Scott,A.D. and May,G.D.
Unpublished (2005)
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