| Library Acc. | MT_IROOT_DSIR |
| Name | Medicago truncatula, roots infected with Phytophthora medicaginis |
| Organism | Medicago truncatula |
| Tissue | roots infected with Phytophthora medicaginis |
| Stage | roots harvested at 10 days post inoculation with Phytophthora medicaginis |
| Description | Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA from roots harvested at 10 days post inoculation with Phytophthora medicaginis. The cDNA was directionally ligated into the Uni-ZAP XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-ZAP phage using Ex-Assist helper phage and propagated in XLOLR cells. Note: EST may be of fungal origin. |
| # of EST/cDNA | 2463 |
| # of Unigene | 1835; Of them, 215 singletons and 1620 TCs. |
| Download (.zip) | link |
| Release Date | Sep 07, 2000 |
| Contact | Contact: Cote F Complex Carbohydrate Research Center University of Georgia 220 Riverbend Road, Athens, GA 30602-4712, USA Tel: 706 542 4451 Fax: 706 542 4412 Email: fcote@ccrc.uga.edu This clone was kindly provided by Carroll Vance; date: 03/22/2000; Submitted to the Database of Expressed Sequence Tags (dbEST) on 05/26/00; More information is available at 'http://chrysie.tamu.edu/medicago'. Seq primer: M13-20 (TGTAAAACGACGGCCAGT). |
| Citation | ESTs for a putative glucan elicitor-binding protein from Medicago truncatula Cote,F., Ojanen-Reuhs,T. and Hahn,M.G. Unpublished (2000) |