Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_JCVI-MT1 |
| Name |
Medicago truncatula, flowers, early seeds, late seeds and stems (JCVI-MT1) |
| Organism |
Medicago truncatula |
| Tissue |
flowers, early seeds, late seeds and stems |
| Stage |
|
| Description |
Vector: pDNR-LIB (Clontech); Site_1: SfiIA; Site_2: SfiIB; Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). Clones were sequenced from the 5' end only. RNA provided by Greg May. This library is also called MTYA. |
| # of EST/cDNA |
8079 |
| # of Unigene |
5454;
Of them, 556 singletons and 4898 TCs.
|
| Download (.zip) |
link |
| Release Date |
Jul 10, 2007 |
| Contact |
Town, Christopher Plant Genomic The J. Craig Venter Institute 9712 Medical Center Dr., Rockville, MD 20850, USA Tel: 301 795 7523 Fax: 301 838 0208 Email: cdtown@jcvi.org For more information about this library see: BMC Genomics. 2006 Oct 24;7:272. This library is also called MTYA. Seq primer: M13F. |
| Citation |
Construction and analysis of normalized cDNA library from flowers, seeds and stems of Medicago truncatula (2007)
Moskal,W., Cheung,F., Xiao,Y. and Town,C.D.
Unpublished (2007)
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