Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_NOD_GVSN |
| Name |
Medicago truncatula, senescent root nodules |
| Organism |
Medicago truncatula |
| Tissue |
senescent root nodules |
| Stage |
mixture of effective nodules from 40 day old plants harvested 36 hours post shoot removal and nodules collected from 2 month old plants at mid-pod stage |
| Description |
Vector: pBluescript SK +/-; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA from the mixture of effective nodules of 40 day old plants harvested 36 hours post shoot removal and nodules collected from 2 month old plants at mid-pod stage. The cDNA was directionally ligated into the Uni-ZAP XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-ZAP phage using Ex-Assist helper phage and propagated in SOLR cells. |
| # of EST/cDNA |
2787 |
| # of Unigene |
1770;
Of them, 257 singletons and 1513 TCs.
|
| Download (.zip) |
link |
| Release Date |
Oct 06, 2000 |
| Contact |
Carroll P. Vance Department of Agronomy and Plant Genetics University of Minnesota 411 Borlaug Hall, 1991 Upper Buford Circle, St.Paul, MN 55108 USA Tel: 612 625 5715 Fax: 651-649-5058 Email: vance004@maroon.tc.umn.edu University of Minnesota name: M273903e TIGR sequence name: MTKBJ96TK More information is available at: http://chrysie.tamu.edu/medicago Seq primer: SKmod (CTA gAA CTA gtg gAT CC). |
| Citation |
ESTs from senescent nodules of Medicago truncatula
Fedorova,M., Pierson,B.L., Samac,D.A., Gantt,J.S., Vance,C.P., Town,C.D., Bowman,C.L., Craven,M.B., Cho,J. and Fraser,C.M.
Unpublished (2000)
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