Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_NOD_GVN |
| Name |
Medicago truncatula, N2-fixing root nodules |
| Organism |
Medicago truncatula |
| Tissue |
N2-fixing root nodules |
| Stage |
effective root nodules harvested one month post inoculation with Sinorhizobium meliloti |
| Description |
Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA from effective root nodules harvested one month post inoculation with Sinorhizobium meliloti. The cDNA was directionally ligated into the Uni-ZAP XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-ZAP phage using Ex-Assist helper phage and propagated in XLOLR cells. |
| # of EST/cDNA |
6468 |
| # of Unigene |
3201;
Of them, 522 singletons and 2679 TCs.
|
| Download (.zip) |
link |
| Release Date |
Apr 11, 2001 |
| Contact |
Carroll P. Vance Department of Agronomy and Plant Genetics University of Minnesota 411 Borlaug Hall, 1991 Upper Buford Circle, St.Paul, MN 55108 USA Tel: 612 625 5715 Fax: 651-649-5058 Email: vance004@maroon.tc.umn.edu University of Minnesota name: M385017e TIGR sequence name: MTCEP96TK More information is available at: http://www.medicago.org Seq primer: SKmod (CTA gAA CTA gtg gAT CC). |
| Citation |
ESTs from one month old nitrogen-fixing root nodules of Medicago truncatula, 2001
Fedorova,M., Pierson,B.L., Samac,D.A., Vance,C.P., Gantt,G.S., Town,C.D., Van Aken,S., Utterback,T., Cho,J. and Fraser,C.M.
Unpublished (2001)
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