Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_ROOTPHOS |
| Name |
Medicago truncatula, roots,phosphate starved |
| Organism |
Medicago truncatula |
| Tissue |
roots |
| Stage |
phosphate starved |
| Description |
Vector: pBluescript SK -; Site_1: EcoRI; Site_2: XhoI; At the trifoliate stage, M. truncatula plants were transplanted to phosphate-free sand and grown for a further 30 days. During this 30 day period, they were fertilized twice weekly with 1/2 Hoaglands solution containing only 20uM potassium phosphate. RNA was prepared from the roots. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in SOLR cells. |
| # of EST/cDNA |
1954 |
| # of Unigene |
1406;
Of them, 148 singletons and 1258 TCs.
|
| Download (.zip) |
link |
| Release Date |
May 02, 2000 |
| Contact |
Contact: Harrison M.J. Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73401 Tel: 580-223-5810 Fax: 580-221-7380 Email: mjharrison@noble.org Date: 1/23/00; Updated to the Database of Expressed Sequence Tags (dbEST) on 04/27/00; More information is available at 'http://chrysie.tamu.edu/medicago'. Seq primer: T3. |
| Citation |
ESTs from phosphate starved roots
Harrison,M.J., Liu,J., Harris,A.R., Scott,A.D., Gonzales,R.A., Gonzales,M.B. and Ellis,L.
Unpublished (1999)
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