Location:
EST Analysis
> EST Library Details
| Library Acc. |
MHRP-root |
| Name |
Medicago truncatula, roots,phosphate-starved |
| Organism |
Medicago truncatula |
| Tissue |
roots |
| Stage |
phosphate-starved |
| Description |
Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; At the trifoliate stage, M. truncatula plants were transplanted to phosphate-free sand and grown for a further 30 days. During this period, they were fertilized twice weekly with 1/2 Hoaglands solutions containing 20uM potassium phosphate. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells. |
| # of EST/cDNA |
2656 |
| # of Unigene |
1799;
Of them, 297 singletons and 1502 TCs.
|
| Download (.zip) |
link |
| Release Date |
Apr 12, 2001 |
| Contact |
Harrison M.J. Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73401 Tel: 580-223-5810 Fax: 580-221-7380 Email: mjharrison@noble.org The Samuel Roberts Noble Foundation: N387937e TIGR sequence name: MTHBJ96TK More information is available at: http://www.medicago.org Seq primer: SKmod (CTA gAA CTA gtg gAT CC). |
| Citation |
ESTs from phosphate-starved roots of Medicago truncatula, 2001
Harrison,M.J., Liu,J., Town,C.D., Van Aken,S., Utterback,T., Cho,J. and Fraser,C.M.
Unpublished (2001)
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