Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_GPOD |
| Name |
Medicago truncatula, immature pod walls |
| Organism |
Medicago truncatula |
| Tissue |
immature pod walls |
| Stage |
Immature pods, ranging in age from 15 to 30 days after pollination |
| Description |
Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; Immature pods, ranging in age from 15 to 30 days after pollination, were collected from greenhouse-grown plants. At harvest, seeds were removed from pods and isolated pod walls were collected and immediately frozen in liquid nitrogen. Pod walls were pooled for mRNA extraction. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propogated in XLOLR cells. |
| # of EST/cDNA |
3620 |
| # of Unigene |
2243;
Of them, 299 singletons and 1944 TCs.
|
| Download (.zip) |
link |
| Release Date |
Jan 21, 2003 |
| Contact |
Grusak, M.A. USDA/ARS Children's Nutrition Research Center Baylor College of Medicine 1100 Bates Street, Houston, TX 77030-2600, USA Tel: 713 798 7044 Fax: 713 798 7078 Email: mgrusak@bcm.tmc.edu TIGR sequence name: MTOBO96TK More information is available at: www.medicago.org Seq primer: SKmod (CTA gAA CTA gtg gAT CC). |
| Citation |
More ESTs from developing reproductive tissues of Medicago truncatula
Grusak,M.A., Samac,D.A., Town,C.D., Van Aken,S., Utterback,T., Cheung,F. and Fraser,C.M.
Unpublished (2002)
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