Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_DSIL |
| Name |
Medicago truncatula, leaves infected with Colletotrichum trifolii,cotyledons and primary leaves harvested 5 and 8 days after inoculation with Colletotrichum trifolii |
| Organism |
Medicago truncatula |
| Tissue |
leaves infected with Colletotrichum trifolii |
| Stage |
cotyledons and primary leaves harvested 5 and 8 days after inoculation with Colletotrichum trifolii |
| Description |
Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA from cotyledons and primary leaves harvested 5 and 8 days after inoculation with Colletotrichum trifolii. The cDNA was directionally ligated into the Uni-ZAP XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-ZAP phage using Ex-Assist helper phage and propagated in XLOLR cells. Note: EST may be of fungal origin. |
| # of EST/cDNA |
6003 |
| # of Unigene |
3212;
Of them, 431 singletons and 2781 TCs.
|
| Download (.zip) |
link |
| Release Date |
Apr 16, 2001 |
| Contact |
Deborah A. Samac Department of Plant Pathology University of Minnesota 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108, USA Tel: 612 625 1243 Fax: 651 649 5058 Email: debbys@puccini.crl.umn.edu University of Minnesota name: M279034e TIGR sequence name: MTFDJ61TK More information is available at: http://chrysie.tamu.edu/medicago Seq primer: SKmod (CTA gAA CTA gtg gAT CC). |
| Citation |
ESTs from leaves and cotyledons of Medicago truncatula after inoculation with Colletotrichum trifolii, 2001
Fedorova,M., Pierson,B.L., Samac,D.A., Vance,C.P., Gantt,G.S., Town,C.D., Van Aken,S., Utterback,T., Cho,J. and Fraser,C.M.
Unpublished (2001)
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