Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_SROOT_KV2 |
| Name |
Medicago truncatula, Seedling roots, inoculation with S. meliloti |
| Organism |
Medicago truncatula |
| Tissue |
Seedling roots |
| Stage |
2 days post-inoculation with Sinorhizobium meliloti |
| Description |
Vector: pBluescript SK -; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in SOLR cells. |
| # of EST/cDNA |
3330 |
| # of Unigene |
2283;
Of them, 391 singletons and 1892 TCs.
|
| Download (.zip) |
link |
| Release Date |
Mar 04, 2002 |
| Contact |
VandenBosch K Department of Plant Biology University of Minnesota 220 BioSci Center, 1445 Gortner Ave, St. Paul, MN 55108, USA Tel: 612 624 2755 Fax: 612 625 1738 Email: kvandenb@cbs.umn.edu TIGR sequence name: MTACK52TK More information is available at: www.medicago.org Seq primer: SKmod (CTA gAA CTA gtg gAT CC). |
| Citation |
ESTs from roots of Medicago truncatula 48 hr after inoculation with Sinorhizobium meliloti
VandenBosch,K., Endre,G., Hur,J., Beremand,P., Town,C.D., Van Aken,S., Utterback,T., Cheung,F., Tsai,J. and Fraser,C.M.
Unpublished (2002)
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