Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_SROOT_KV0 |
| Name |
Medicago truncatula, Seedling roots |
| Organism |
Medicago truncatula |
| Tissue |
Seedling roots |
| Stage |
Immediately prior to inoculation with Sinorhizobium meliloti (0 hour) |
| Description |
Vector: pBluescript SK -; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells. |
| # of EST/cDNA |
2752 |
| # of Unigene |
2031;
Of them, 298 singletons and 1733 TCs.
|
| Download (.zip) |
link |
| Release Date |
Apr 16, 2001 |
| Contact |
VandenBosch K Department of Plant Biology University of Minnesota 220 BioSci Center, 1445 Gortner Ave, St. Paul, MN 55108, USA Tel: 612 624 2755 Fax: 612 625 1738 Email: kvandenb@cbs.umn.edu Texas A&M University name:T266713e TIGR sequence name: MTGAL95TK More information is available at: http://chrysie.tamu.edu/medicago Seq primer: SKmod (CTA gAA CTA gtg gAT CC). |
| Citation |
ESTs from uninoculated seedling roots of Medicago truncatula, 2001
VandenBosch,K., Endre,G., Hur,J., Moore,J., Beremand,P., Town,C.D., Van Aken,S., Utterback,T., Cho,J. and Fraser,C.M.
Unpublished (2001)
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