Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_SIRRA |
| Name |
Medicago truncatula, seedlings |
| Organism |
Medicago truncatula |
| Tissue |
seedlings |
| Stage |
seedling |
| Description |
Vector: Lambda Zap; Seedlings were exposed either to 100 gy gamma or 0.5, 1, 5, or 10 kJ/m2 UV irradiation. Gamma-irradiated samples were harvested at 6, 12, 24 and 48 hours after treatment. UV-irradiated samples were harvested 24 hours post-treatment. cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each sample. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells. |
| # of EST/cDNA |
5992 |
| # of Unigene |
2491;
Of them, 664 singletons and 1827 TCs.
|
| Download (.zip) |
link |
| Release Date |
Apr 24, 2002 |
| Contact |
May GD Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73402, USA Tel: 580 224 6650 Fax: 580 224 6692 Email: gdmay@noble.org Insert Length: 471 Std Error: 0.00 Plate: 108 row: H column: 08 Seq primer: TCACACAGGAAACAGCTATGAC. |
| Citation |
Expressed Sequence Tags from the Samuel Roberts Noble Foundation Medicago truncatula irradiated library
Torres-Jerez,I., Scott,A.D., Harris,A.R., Gonzales,R.A., Bell,C.J., Flores,H.R., Inman,J.T., Weller,J.W. and May,G.D.
Unpublished (2001)
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