Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_VILEAF |
| Name |
Medicago truncatula,AMV-virus-infected leaves |
| Organism |
Medicago truncatula |
| Tissue |
Virus-infected leaves |
| Stage |
AMV infected leaves were harvested at 14 & 17dpi |
| Description |
Vector: Lambda Zap; Medicago truncatula leaves were infected with alfalfa mosaic virus (AMV). AMV infected leaves were harvested at 14 & 17dpi. Equal amounts of total RNA were isolated and pooled for mRNA purification. RNA for cDNA library construction was isolated from the pooled tissues. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells. |
| # of EST/cDNA |
7086 |
| # of Unigene |
2236;
Of them, 185 singletons and 2051 TCs.
|
| Download (.zip) |
link |
| Release Date |
Jan 11, 2005 |
| Contact |
May GD Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73402, USA Tel: 580 224 6650 Fax: 580 224 6692 Email: gdmay@noble.org. |
| Citation |
Medicago truncatula Alfalfa Mosaic Virus- (AMV) Infected Leaves Expressed Sequence Tags from the Samuel Roberts Noble Foundation - Center for Medicago Genomics Research
Liu,J., Scott,A.D., May,G.D. and Nelson,R.S.
Unpublished (2005)
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