Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_HOGA |
| Name |
Medicago truncatula, seedling roots treated with oligogalacturonides (DP 6-20) |
| Organism |
Medicago truncatula |
| Tissue |
3 day old seedling roots |
| Stage |
24 hours after treatment in the dark at 26 C with 0.5 mg/ml oligogalacturonides (DP 6-20) in the presence of 100 ug/ml Gentamicin |
| Description |
Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in SOLR cells. |
| # of EST/cDNA |
5794 |
| # of Unigene |
3546;
Of them, 797 singletons and 2749 TCs.
|
| Download (.zip) |
link |
| Release Date |
Apr 24, 2003 |
| Contact |
Michael G. Hahn Complex Carbohydrate Research Center University of Georgia 220 Riverbend Road, Athens, GA 30602-4712, USA Tel: 706-542-4457 Fax: 706-542-4412 Email: hahn@ccrc.uga.edu TIGR sequence name: MTMED96TK More information is available at: www.medicago.org Seq primer: SKmod (CTA gAA CTA gtg gAT CC). |
| Citation |
ESTs from roots of Medicago truncatula treated with oligogalacturonides of DP 6-20
Hahn,M.G., Ojanen-Reuhs,T., Samac,D., Town,C.D., Van Aken,S., Utterback,T., Cho,J. and Fraser,C.M.
Unpublished (2001)
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