Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_SROOT_KV1 |
| Name |
Medicago truncatula, Seedling roots, inoculation with S. meliloti |
| Organism |
Medicago truncatula |
| Tissue |
Seedling roots |
| Stage |
24 hours post-inoculation with Sinorhizobium meliloti |
| Description |
Vector: pBluescript SK -; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells. |
| # of EST/cDNA |
2840 |
| # of Unigene |
1962;
Of them, 349 singletons and 1613 TCs.
|
| Download (.zip) |
link |
| Release Date |
Oct 06, 2000 |
| Contact |
VandenBosch K Department of Plant Biology University of Minnesota 220 BioSci Center, 1445 Gortner Ave, St. Paul, MN 55108, USA Tel: 612 624 2755 Fax: 612 625 1738 Email: kvandenb@cbs.umn.edu Texas A&M University name: T268675e TIGR sequence name: MTIBJ95TK More information is available at: http://chrysie.tamu.edu/medicago Seq primer: SKmod (CTA gAA CTA gtg gAT CC). |
| Citation |
ESTs from roots of Medicago truncatula 24 hours after inoculation with Sinorhizobium meliloti
VandenBosch,K., Endre,G., Hur,J., Moore,J., Beremand,P., Ellis,L., Town,C.D., Bowman,C.L., Craven,M.B., Hansen,T.S., Holt,I.E. and Fraser,C.M.
Unpublished (1999)
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