Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_KVKC |
| Name |
Medicago truncatula, mixed tissues |
| Organism |
Medicago truncatula |
| Tissue |
mixed tissues |
| Stage |
various stages |
| Description |
Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells. |
| # of EST/cDNA |
1671 |
| # of Unigene |
1094;
Of them, 85 singletons and 1009 TCs.
|
| Download (.zip) |
link |
| Release Date |
Jul 29, 2002 |
| Contact |
VandenBosch K Department of Plant Biology University of Minnesota 220 BioSci Center, 1445 Gortner Ave, St. Paul, MN 55108, USA Tel: 612 624 2755 Fax: 612 625 1738 Email: kvandenb@cbs.umn.edu Likely Clone_id: pKVKC-12H8 TIGR sequence name: MTNAL92TV More information is available at: www.medicago.org Seq primer: (gtA AtA CgA CtC ACt AtA ggg C). |
| Citation |
The Medicago truncatula 'kiloclone' set; ESTs selected and re-arrayed from various libraries
VandenBosch,K., Endre,G., Silverstein,K, Town,C.D., Van Aken,S., Utterback,T., Cheung,F. and Fraser,C.M.
Unpublished (2002)
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