Location:
EST Analysis
> EST Library Details
| Library Acc. |
MT_JCVI-MT3 |
| Name |
JCVI-MT3 |
| Organism |
Medicago truncatula |
| Tissue |
mixed root and nodules |
| Stage |
|
| Description |
Vector: pDNR-LIB (Clontech); Site_1: SfiIA; Site_2: SfiIB; Tissue: low-phosphate treated root, Glomus versiforme infected root, uninoculated root, Sinorhizobium meliloti T202 inoculated root and nodules (3-11 DAI), Sinorhizobium meliloti 102F51 inoculated root and nodules (3-21 DAI), Sinorhizobium meliloti 1021 inoculated root and nodules (28 DAI). Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (http://www.evrogen.com/t2.shtml). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease (DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). Clones were sequenced from the 5' end only. RNA provided by Maria Harrison, Sue Miller, Carrol Vance and Vagner Benedito. |
| # of EST/cDNA |
4966 |
| # of Unigene |
3806;
Of them, 573 singletons and 3233 TCs.
|
| Download (.zip) |
link |
| Release Date |
Nov 27, 2007 |
| Contact |
Chan, Agnes Plant Genomics The J. Craig Venter Institute 9704 Medical Center Dr., Rockville, MD 20850, USA Tel: 301 795 7862 Fax: 301 838 0208 Email: achan@jcvi.org This library is also called META. Seq primer: M13F. |
| Citation |
Construction and analysis of normalized cDNA library from root tissues of Medicago truncatula
Moskal,W., Chan,A.P. and Town,C.D.
Unpublished (2007)
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