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EST Analysis
> EST Library Details
| Library Acc. |
MT_JCVI-MT2 |
| Name |
JCVI-MT2 |
| Organism |
Medicago truncatula |
| Tissue |
mixed |
| Stage |
|
| Description |
Vector: pDNR-LIB (Clontech); Site_1: SfiIA; Site_2: SfiIB; Tissues: drought-stressed leaves, roots and nodules (8 weeks post-inoculation with S. meliloti 2011); fungal-infected roots (3, 5, 7 days post-inoculation with P. omnivora of 4-week-old plant); salt-treated leaves and roots (1, 6 hours post-treatment with 100mM NaCl); cell culture (root-derived cell suspension with equal parts of unelicited and yeast-elicited cell cultures at 3 hrs post-elicitation), and untreated aerial tissues including cotyledons, terminal buds, leaves, petioles and developing pods. Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (http://www.evrogen.com/technologies/SMART.shtml). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease (DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). RNA provided by Esther Gonzalez, Rao Uppalapati, Sue Miller, Carrol Vance, Florian Frugier, Vagner Benedito and Greg May. |
| # of EST/cDNA |
9379 |
| # of Unigene |
4010;
Of them, 395 singletons and 3615 TCs.
|
| Download (.zip) |
link |
| Release Date |
Oct 16, 2008 |
| Contact |
Chan, Agnes Plant Genomics The J. Craig Venter Institute 9704 Medical Center Dr., Rockville, MD 20850, USA Tel: 301 795 7862 Fax: 301 838 0208 Email: achan@jcvi.org This library is also called MEUA. Seq primer: M13R. |
| Citation |
Construction and analysis of normalized cDNA library from stressed or untreated aerial tissues of Medicago truncatula
Chan,A.P., Cheung,F., Xiao,Y. and Town,C.D.
Unpublished (2008)
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