Location:
EST Analysis
> EST Library Details
| Library Acc. |
MS_TRI1 |
| Name |
Medicago sativa(Alfalfa) trichomes(2006) |
| Organism |
Medicago sativa |
| Tissue |
Glandular trichomes isolated from stem |
| Stage |
Trichomes were removed from internodes of 8-12 inch tall stems |
| Description |
Vector: pDNR-LIB; Glandular-haired alfalfa plants were established in a Conviron growth chamber (16-h days, full lights, 24oC set point) in 40 one gallon pots. Plants were grown in Metromix 350 and fertilized with MiracleGro as needed. They were cut back closely to encourage the emergence of vigorous shoots. Trichomes were isolated from stems, approximately 8-12 inches long, clipped from plants 2-3 inches above the crown. With minimal handling of the stem, the apical bud, leaves and nodes were discarded, and the trichomes isolated from the internode segments. |
| # of EST/cDNA |
5275 |
| # of Unigene |
2811;
Of them, 1968 singletons and 843 TCs.
|
| Download (.zip) |
link |
| Release Date |
Jun 20, 2006 |
| Contact |
Name: May GD
Lab: Plant Biology Division
Institution: The Samuel Roberts Noble Foundation
Address: 2510 Sam Noble Parkway, Ardmore, OK 73402, USA
Tel: 580 224 6650
Fax: 580 224 6692
E-mail: gdmay@noble.org |
| Citation |
Transcriptome analysis of alfalfa glandular trichomes
Aziz,N., Paiva,N.L., May,G.D. and Dixon,R.A.
Planta 221 (1), 28-38 (2005)
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