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EST Analysis
> EST Library Details
| Library Acc. |
MS_TRI2 |
| Name |
Alfalfa (Medicago sativa) Trichome isolated from the stem |
| Organism |
Medicago sativa |
| Tissue |
Glandular trichomes isolated from stem |
| Stage |
Trichome from the stem, approximately 8-12 inches long, clipped from plants 2-3 inches above the crown |
| Description |
Vector: pDNR-LIB; Site_1: Sfi IA (GGCCATTACGGCC); Site_2: Sfi IB (GGCCGCCTCGGCC); Glandular-haired alfalfa plants were established in green house in 40 half gallon pots. Plants were grown in Metromix 350 and fertilized with MiracleGro as needed. They were cut back closely to encourage the emergence of vigorous shoots. Trichomes were isolated from stems, approximately 8-12 inches long, clipped from plants 2-3 inches above the crown. With minimal handling of the stem, the apical bud, leaves and nodes were discarded, and the trichomes isolated from the internode segments. The cDNA library from alfalfa trichome was constructed using the Creator Smart cDNA library construction kit (Clontech, Palo Alto, CA, USA) following the manufacturer's protocol for total RNA. |
| # of EST/cDNA |
4384 |
| # of Unigene |
2155;
Of them, 1438 singletons and 717 TCs.
|
| Download (.zip) |
link |
| Release Date |
Oct 09, 2007 |
| Contact |
Zhao Xuechun Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73402, USA Tel: 580 224 6725 Fax: 580 224 6692 Email: pzhao@noble.org. |
| Citation |
Analysis of cDNA libraries from trichome of Alfalfa (Medicago sativa)
Wang,G., Aziz,N., King,A., Zhao,X. and Dixon,R.A.
Unpublished (2007)
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