Location:
EST Analysis
> EST Library Details
| Library Acc. |
NT_TRI |
| Name |
Burley21 trichome library |
| Organism |
Nicotiana tabacum |
| Tissue |
trichome |
| Stage |
|
| Description |
Organ: Leaf; Plants were grown on soil in glasshouses under long days (16h:8h). RNA was extracted from trichomes based on the method of Aziz et al. (2004). Briefly, stems from 30 plants were cut into 5-10cm fragments and flash frozen in liquid nitrogen. Segments were individually shaken in pre-frozen 50ml platic tubes to release the trichomes. Collected trichomes were stored in Tri Reagent (Sigma) and RNA was extracted according to manufacturers instructions. |
| # of EST/cDNA |
1949 |
| # of Unigene |
2095;
Of them, 967 singletons and 1128 TCs.
|
| Download (.zip) |
link |
| Release Date |
Aug 14, 2008 |
| Contact |
Edwards, K.D. Advanced Technologies (Cambridge) ATC Ltd, 210 Cambridge Science Park, Cambridge, UK
Tel: +441223429502 Fax: +441223423448. |
| Citation |
Extending Genomic Resources for Nicotiana tabacum
Coates,S.A., Dorlac de Borne,F., Edwards,K.D., Ross,J., Story,G.W., Yang,A.W. and Jones,L.
Unpublished (2008)
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