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EST Analysis
> EST Library Details
| Library Acc. |
AA_GTRI5 |
| Name |
CATG Artemisia annua, Tanzanian, from Sandeman Seeds, 2+kb |
| Organism |
Artemisia annua |
| Tissue |
glandular trichomes with 10-20% palisade cells and hair trichomes |
| Stage |
Transition to flowering |
| Description |
Vector: pDNR-LIB; Site_1: SfiI A; Site_2: SfiI B; The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created by the Keasling Research Group, University of California, Berkeley, and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA). |
| # of EST/cDNA |
4264 |
| # of Unigene |
3718;
Of them, 2963 singletons and 755 TCs.
|
| Download (.zip) |
link |
| Release Date |
Nov 09, 2007 |
| Contact |
Lindquist,E.A., Richardson,P. DOE Joint Genome Institute 2800 Mitchell Drive, Walnut Creek, CA 94598, USA Tel: 925 296 5600 Fax: 925 296 5710 Email: cdna@jgi-psf.org Tissue Procurement: Keasling Research Group, UC Berkeley cDNA Library Preparation: Keasling Research Group, UC Berkeley cDNA Library Arrayed by: DOE Joint Genome Institute: http://www.jgi.doe.gov DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov Clone Distribution: For inquiries regarding access to clones:Jay Keasling (keasling@berkeley.edu) Plate: CATG 0025 row: C column: 16. |
| Citation |
DOE Joint Genome Institute Artemisia annua EST project
Newman,K.L., Keasling,J.D., Richardson,P., Lucas,S., Rokhsar,D., Wang,M. and Lindquist,E.A.
Unpublished (2007)
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