Location:
EST Analysis
> EST Library Details
| Library Acc. |
NB_SAL_US |
| Name |
Nicotiana benthamiana, expanding leaf tissue |
| Organism |
Nicotiana benthamiana |
| Tissue |
expanding leaf tissue |
| Stage |
|
| Description |
Vector: pBluescript S/K +; Site_1: EcoR1; Site_2: Xho1; Total RNA isolated from tissue, poly A fraction isolated on oligo-dT column. mRNA reverse transcribed and double stranded. Poly A dsRNA ranging in size from 400-4000 bp directionally cloned into EcoR1 and Xho1 restricted into pBluescript. EcoR1 site at the 5' end of the cDNA and Xh01 on the 3' side. |
| # of EST/cDNA |
8086 |
| # of Unigene |
2702;
Of them, 1911 singletons and 791 TCs.
|
| Download (.zip) |
link |
| Release Date |
Sep 08, 2004 |
| Contact |
Dr. Steven A. Lommel Tobacco Genome Initiative North Carolina State University Box 7253, NCSU, Raleigh, NC 27606, USA Tel: 9195130006 Fax: 9195159500 Email: steve_lommel@ncsu.edu Homology: e-val = 1.4e-070. Description = tryptophan synthase, beta subunit 1 (TSB1) [Arabidopsis thaliana] gi|136251|sp|P14671|TRP1_ARATH Tryptophan synthase beta chain 1, chloroplast precursor gi|99767|pir|A31393 tryptophan synthase (EC 4.2.1.20) beta-1 chain precursor - Arabidopsis thaliana Homology: e-val = 2.7e-068. Description = Tryptophan synthase beta chain 2, chloroplast precursor gi|2792520|gb|AAB97087.1| tryptophan synthase beta subunit [Camptotheca acuminata] gi|2801771|gb|AAB97526.1| tryptophan synthase beta [Camptotheca acuminata] row: d column: 22 High quality sequence stop: 647. |
| Citation |
Tobacco Genome Initiative (TGI) Nicotiana benthamiana ESTs
Opperman,C.H., Lommel,S.A,, Burke,M., Feulner,G., Carlson,J., George,C., Gove,S., Houfek,T.D., Jefferys,S.R., Kalat,S., King,R., Levin,J., Little,P.C., Lumpkin,A., Ross,T., Salstead,A., Scholl,E.H., Sosinski,B.R., Stephens,P.J. and Zekanis,S.H.
Unpublished (2004)
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