Location:
EST Analysis
> EST Library Details
| Library Acc. |
LIBEST_024544 |
| Name |
LIBEST_024544 NBREL2 |
| Organism |
Nicotiana benthamiana |
| Tissue |
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| Stage |
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| Description |
Organ: Root; Vector: pYL156; Site_1: BamH1; Site_2: EcoR1; The library NBREL2 was constructed using the suppression subtractive hybridization technique (Clontech PCR-select subtractive cDNA library kit) with RNA isolated from hydroponically-grown Nicotiana benthamiana plants as previously described (Liu & Page (2008) Plant Methods 4: 5). NBREL2 was created using mRNA from methyl jasmonate-treated roots as tester and untreated roots as driver. The subtracted cDNAs were amplified using primers containing BamH1 and EcoR1 sites, cloned directly into the TRV-RNA2 plasmid pYL156 (AF406991) and electroporated into Agrobacterium tumefaciens. cDNA inserts amplified from A. tumefaciens cultures were used as sequencing templates. |
| # of EST/cDNA |
1424 |
| # of Unigene |
1057;
Of them, 794 singletons and 263 TCs.
|
| Download (.zip) |
link |
| Release Date |
Apr 26, 2009 |
| Contact |
Jonathan Page NRC Plant Biotechnology Institute 110 Gymnasium Place, Saskatoon, Saskatchewan, S7N 0W9, Canada Tel: 306-975-4187 Fax: 306-975-4839 Email: jon.page@nrc-cnrc.gc.ca. |
| Citation |
Functional Genomics of Alkaloid Biosynthesis in Nicotiana benthamiana
Li,R. and Page,J.E.
Unpublished (2009)
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