Location:
EST Analysis
> EST Library Details
| Library Acc. |
HLUPLC |
| Name |
HLUPLC |
| Organism |
Humulus lupulus |
| Tissue |
Trichome (lupulin gland) |
| Stage |
Trichomes isolated from mid to ripe stage hop cones |
| Description |
Vector: pBluescript SK+; Site_1: EcoRI; Site_2: XhoI; The library HLUPLC was constructed from RNA isolated from lupulin glands from the variety 'Taurus'. Hop cones were collected near Mainburg, Germany in August 2003. Lupulin glands were isolated by agitating dissected cones in liquid nitrogen followed by filtration to remove cone material. Total RNA was isolated using an RNeasy Plant Mini Kit (Qiagen) and mRNA was purified using a Dynabeads mRNA purification kit (Qiagen). HLUPLC was constructed using a cDNA library synthesis kit (Stratagene) and cDNAs ligated into the plasmid vector pBluescript SK+. The library was normalized using a hydroxyapatite-based method (C0t 2.5 and 5) (Bonaldo et al. (1996) Genome Res. 6, 791). |
| # of EST/cDNA |
2370 |
| # of Unigene |
1312;
Of them, 221 singletons and 1091 TCs.
|
| Download (.zip) |
link |
| Release Date |
Sep 17, 2008 |
| Contact |
Jonathan Page NRC Plant Biotechnology Institute 110 Gymnasium Place, Saskatoon, Saskatchewan, S7N 0W9, Canada Tel: 306-975-4187 Fax: 306-975-4839 Email: jon.page@nrc-cnrc.gc.ca. |
| Citation |
EST analysis of hop glandular trichomes identifies an O-methyltransferase that catalyzes the biosynthesis of xanthohumol
Nagel,J., Culley,L., Lu,Y., Matthews,P.D., Stevens,J.F. and Page,J.E.
Plant Cell 20 (1), 186-200 (2008)
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