Location:
EST Analysis
> EST Library Details
| Library Acc. |
SL_ROOTPHOS |
| Name |
Solanum lycopersicum, root,5 weeks old plants |
| Organism |
Solanum lycopersicum |
| Tissue |
root |
| Stage |
5 weeks old plants |
| Description |
Vector: PGEM Teasy; Tomato plants were grown hydroponically in modified Hoagland's solution containing 0.5 mM of KH2PO4. 4-week-old plants were subjected for phosphate starvation treatment by omitting the KH2PO4 from the nutrient solution. Tomato roots were harvested after 7-day phosphate starvation. Total and poly A+ RNA was isolated from phosphate starvation and control roots, respectively. Subtractive cDNA library was constructed using PCR-Select cDNA subtraction Kit (Clontech) to selectively amplify up-regulated genes under phosphate starvation condition. |
| # of EST/cDNA |
823 |
| # of Unigene |
602;
Of them, 255 singletons and 347 TCs.
|
| Download (.zip) |
link |
| Release Date |
Oct 25, 2006 |
| Contact |
Contact: Tzyy-Jen Chiou Tzyy-Jen Chiou Institute of BioAgricultural Sciences, Academia Sinica Nankang, Taipei, 115, Taiwan, Republic of China Tel: 886-2-26538853 Fax: 886-2-26515600 Email: tjchiou@gate.sinica.edu.tw Insert Length: 839 Std Error: 0.00 Plate: 01 row: C column: 05 Seq primer: M13F. |
| Citation |
Isolation and characterization of tomato Hsa32 encoding a novel heat-shock protein
Liu,N.Y., Ko,S.S., Yeh,K.C. and Charng,Y.Y.
Plant Sci. 170 (5), 976-985 (2006)
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