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EST Analysis
> EST Library Details
| Library Acc. |
SL_pericarp |
| Name |
Solanum lycopersicum, Pericarp,Turning stage of fruit ripening. |
| Organism |
Solanum lycopersicum |
| Tissue |
Pericarp |
| Stage |
Turning stage of fruit ripening. |
| Description |
Equal amounts of total RNA isolated from two tomato genotypes differing in juice Bostwick value were mixed and used to prepare a normalized cDNA library (Takahashi-N and Ko-MSH, 1994: Toward a whole cDNA catalog: construction of an equalized cDNA library from mouse embryos. Genomics-San-Diego, 23: 1, 202-210). The resultant PCR amplified products were ligated into pBluscript SKII- vector (Stratagene) and transformed into E. coli DH5alpha |
| # of EST/cDNA |
1529 |
| # of Unigene |
1450;
Of them, 405 singletons and 1045 TCs.
|
| Download (.zip) |
link |
| Release Date |
May 02, 2003 |
| Contact |
Handa, AK Department of Horticulture and Landscape Architecture Purdue University 625 Agriculture Mall Drive, West Lafayette, IN 47907-2010,USA Tel: 765 494 1339 Fax: 765 494 0391 Email: handa@hort.purdue.edu Plate: RR2_C03_R.nr Seq primer: T3. |
| Citation |
Generation of a set of tomato cDNA clones from turning stage of fruits for macroarray analyses
Srivastava,A., Datsenka,T., Kudla,K., Bhaskaran,A. and Handa,A.K.
Unpublished (2003)
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