Location:
EST Analysis
> EST Library Details
| Library Acc. |
CS_GT |
| Name |
Cannabis sativa glandular trichomes |
| Organism |
Cannabis sativa marijuana cultivar Skunk #1 |
| Tissue |
Glandular trichome from female inflorescences |
| Stage |
various developmental stages |
| Description |
Vector: pDNR-LIB; Site_1: Sfi IA (GGCCATTACGGCC); Site_2: Sfi IB (GGCCGCCTCGGCC); Cannabis sativa plants were grown and processed in a DEA licensed and secured laboratory. Glands were removed from female inflorescences by vortexing, selected by sieving through a screen mesh, and collected by centrifugation. Total RNA was isolated from the glands using the Qiagen RNAeasy Plant Mini Kit (Qiagen Inc.-USA, Valencia, CA). The cDNA library from Cannabis trichomes was constructed using the Creator Smart cDNA library construction kit (Clontech, Palo Alto, CA, USA) following the manufacturer's protocol for total RNA. |
| # of EST/cDNA |
1540 |
| # of Unigene |
1271;
Of them, 1155 singletons and 116 TCs.
|
| Download (.zip) |
link |
| Release Date |
May 21, 2010 |
| Contact |
Marks, M. David Department of Plant Biology University of Minnesota 1445 Gortner Ave Rm 250, St. Paul, MN 55108, USA Tel: 612-625-6737 Fax: 612-625-1738 Email: marks004@umn.edu. |
| Citation |
Identification of candidate genes affecting Delta9-tetrahydrocannabinol biosynthesis in Cannabis sativa
Marks,M.D., Tian,L., Wenger,J.P., Omburo,S.N., Soto-Fuentes,W., He,J., Gang,D.R., Weiblen,G.D. and Dixon,R.A.
J. Exp. Bot. 60 (13), 3715-3726 (2009)
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