Location:
EST Analysis
> EST Library Details
| Library Acc. |
LIBEST_025694 |
| Name |
LIBEST_025694 GSTSUB |
| Organism |
Artemisia annua |
| Tissue |
glandular trichome minus flower bud |
| Stage |
19-21 days after induction of flowering |
| Description |
Organ: flower bud; Vector: pDNR-LIB; Plants (seed source: Elixir Farm Botanicals, Brixey,MO, USA) were grown with 16 h/25 C days and 8 h/20 C nights to a height of approximately 1.2 m (about 3 months) then transferred to 12 h/25 C days and 12 h/20 C nights. Glandular trichomes were isolated by abrasion using a Bead Beater (Biospec) and filtration through nylon mesh. A subtracted cDNA library was constructed with a PCR-Select cDNA Subtraction Kit (Clontech) using poly A+ RNA isolated from glandular trichomes (tester) and flower buds (driver). |
| # of EST/cDNA |
3525 |
| # of Unigene |
1685;
Of them, 1315 singletons and 370 TCs.
|
| Download (.zip) |
link |
| Release Date |
May 21, 2010 |
| Contact |
Covello PS National Research Council Canada Plant Biotechnology Institute 110 Gymnasium Place, Saskatoon, SK, Canada S7N 0W9 Tel: 306 975 5269 Fax: 306 975 4839 Email: patrick.covello@nrc-cnrc.gc.ca. |
| Citation |
Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin
Teoh,K.H., Polichuk,D.R., Reed,D.W., Nowak,G. and Covello,P.S.
FEBS Lett. 580 (5), 1411-1416 (2006)
|
|
