Location:
EST Analysis
> EST Library Details
| Library Acc. |
LIBEST_025693 |
| Name |
LIBEST_025693 AAGST |
| Organism |
Artemisia annua |
| Tissue |
glandular trichome |
| Stage |
19-21 days after induction of flowering |
| Description |
Organ: flower bud; Vector: pDNR-LIB; Plants (seed source: Elixir Farm Botanicals, Brixey,MO, USA) were grown with 16 h/25 C days and 8 h/20 C nights to a height of approximately 1.2 m (about 3 months) then transferred to 12 h/25 C days and 12 h/20 C nights. Glandular trichomes were isolated by abrasion using a Bead Beater (Biospec) and filtration through nylon mesh. cDNA synthesis and library construction were carried out with a Creator SMART cDNA Library Construction Kit (Clontech) using Long-Distance PCR. |
| # of EST/cDNA |
3690 |
| # of Unigene |
2276;
Of them, 1473 singletons and 803 TCs.
|
| Download (.zip) |
link |
| Release Date |
May 21, 2010 |
| Contact |
Covello PS National Research Council Canada Plant Biotechnology Institute 110 Gymnasium Place, Saskatoon, SK, Canada S7N 0W9 Tel: 306 975 5269 Fax: 306 975 4839 Email: patrick.covello@nrc-cnrc.gc.ca. |
| Citation |
Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin
Teoh,K.H., Polichuk,D.R., Reed,D.W., Nowak,G. and Covello,P.S.
FEBS Lett. 580 (5), 1411-1416 (2006)
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