Location:
EST Analysis
> EST Library Details
| Library Acc. |
LIBEST_025264 |
| Name |
LIBEST_025264 LA1777 Type I trichomes |
| Organism |
Solanum habrochaites |
| Tissue |
Type I trichomes |
| Stage |
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| Description |
Handpicked type I glands from leaves of growth chamber grown Solanum habrochaites LA1777 plants (25oC for 16/8 h photoperiod). Total RNA was extracted from 2000 handpicked type I trichomes from the leaf tissue of Solanum habrochaites LA1777 plants and used directly for cDNA synthesis using the Clontech Super SMART cDNA synthesis kit according to kit protocols but with modified primers. Primers used in the construction of this library: RT1: 5' -TAGAGGCCGAGGCGGCCGACATGTTTTGTTTTTTTTTCTTTTTTTTTTVN-3'; RT2: 5' CGCAAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'; 5' PCR primer: 5' -AAGCAGTGGTATCAACGCAGAGT-3'; 3' PCR primer: 5' -TAGAGGCCGAGGCGGCCGAC-3' |
| # of EST/cDNA |
1004 |
| # of Unigene |
970;
Of them, 447 singletons and 523 TCs.
|
| Download (.zip) |
link |
| Release Date |
Jan 06, 2011 |
| Contact |
Last R. L. Department of Biochemistry and Molecular Biology and Plant Biology Michigan State University 301 Biochemistry Building, East Lansing, MI 48824, USA Tel: (517) 432-3278 Fax: (517) 353-9334 Email: lastr@msu.edu. |
| Citation |
Comparative Functional Genomic Analysis of Solanum Glandular Trichome Types
McDowell,E.T., Kaptyen,J., Schmidt,A., Li,C., Kang,J.H., Descour,A., Shi,F., Larson,M., Schilmiller,A., An,L., Jones,A.D., Pichersky,E., Soderlund,C.A. and Gang,D.R.
Plant Physiol. 155 (1), 524-539 (2011)
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