Location:
EST Analysis
> EST Library Details
| Library Acc. |
AA_CAIY |
| Name |
CAIY Artemisia annua leaf |
| Organism |
Artemisia annua |
| Tissue |
leaf |
| Stage |
|
| Description |
Vector: pMCL200_cDNA; Site_1: SalI; Site_2: NotI; The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.5-3kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cdna vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA). |
| # of EST/cDNA |
12984 |
| # of Unigene |
5411;
Of them, 3612 singletons and 1799 TCs.
|
| Download (.zip) |
link |
| Release Date |
Nov 09, 2007 |
| Contact |
Lindquist,E.A., Richardson,P. DOE Joint Genome Institute 2800 Mitchell Drive, Walnut Creek, CA 94598, USA Tel: 925 296 5600 Fax: 925 296 5710 Email: cdna@jgi-psf.org cDNA Library Preparation: DOE Joint Genome Institute: http://www.jgi.doe.gov cDNA Library Arrayed by: DOE Joint Genome Institute: http://www.jgi.doe.gov DNA Sequencing: DOE Joint Genome Institute http://www.jgi.doe.gov Clone Distribution: For inquiries regarding access to clones:Jay Keasling (keasling@berkeley.edu) Plate: CAIY 0025 row: I column: 4. |
| Citation |
DOE Joint Genome Institute Artemisia annua EST project
Newman,K.L., Keasling,J.D., Richardson,P., Lucas,S., Rokhsar,D., Wang,M. and Lindquist,E.A.
Unpublished (2007)
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