Location:
EST Analysis
> EST Library Details
| Library Acc. |
LIBEST_025327 |
| Name |
LIBEST_025327 two celled proembryo suppression subtractive cDNA library |
| Organism |
Nicotiana tabacum |
| Tissue |
embryo |
| Stage |
two-celled proembryo |
| Description |
Organ: embryo; Vector: pGEMT Easy; cDNA of the apical and basal cells from tobacco two-celled proembryo was subtracted and cloned into vector for screening, then the identified clones was sequenced for EST analysis. |
| # of EST/cDNA |
794 |
| # of Unigene |
319;
Of them, 79 singletons and 240 TCs.
|
| Download (.zip) |
link |
| Release Date |
Nov 02, 2010 |
| Contact |
Jie Zhao Key Laboratory of the Ministry of Education for Plant Developmental Biology College of Life Sciences, Wuhan University Luojia Hill, WuChang, Wuhan, Hubei 430072, China Tel: +86-27-68756010 Fax: +86-27-68756010 Email: jzhao@whu.edu.cn Seq primer: T7 or SP6. |
| Citation |
Comparative transcriptional profiling analysis of the two daughter cells from tobacco zygote reveals the transcriptome differences in the apical and basal cells
Hu,T.X., Yu,M. and Zhao,J.
BMC Plant Biol. 10, 167 (2010)
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