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EST Analysis
> EST Library Details
| Library Acc. |
LIBEST_026715 |
| Name |
LIBEST_026715 Tobacco two-celled proembryo cDNA library |
| Organism |
Nicotiana tabacum |
| Tissue |
|
| Stage |
after first zygote division |
| Description |
Vector: pTripEX2; Site_1: Sfi IA; Site_2: Sfi IB; Two-celled proembryo were manually collected from ovules. RNA was isolated and cDNA library was constructed by using the Clontech SMART cDNA library construction kit. The cDNAs were directionally ligated into pTripEX2. |
| # of EST/cDNA |
2149 |
| # of Unigene |
1633;
Of them, 325 singletons and 1308 TCs.
|
| Download (.zip) |
link |
| Release Date |
Jul 14, 2011 |
| Contact |
Haiping Xin Key Laboratory of the Ministry of Education for Plant Developmental Biology College of Life Science, Wuhan University Box3012, Hubei, 430072 China Tel: 862768756170 Fax: 862768756010 Email: xinhaiping215@hotmail.com Seq primer: pTripEX2 5' sequencing primer. |
| Citation |
Dynamic changes of transcript profiles after fertilization are associated with de novo transcription and maternal elimination in tobacco zygote, and mark the onset of the maternal-to-zygotic transition
Zhao,J., Xin,H., Qu,L., Ning,J., Peng,X., Yan,T., Ma,L., Li,S. and Sun,M.X.
Plant J. 65 (1), 131-145 (2011)
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