Location:
EST Analysis
> EST Library Details
| Library Acc. |
LIBEST_026545 |
| Name |
LIBEST_026545 Tobacco sperm cell cDNA library |
| Organism |
Nicotiana tabacum |
| Tissue |
|
| Stage |
Before fertilization |
| Description |
Vector: pTripEX2; Site_1: Sfi IA; Site_2: Sfi IB; Sperm cells were manually collected from pollen tubes. RNA was isolated and cDNA library was constructed by using the Clontech SMART cDNA library construction kit. The cDNAs were directionally ligated into pTripEX2. |
| # of EST/cDNA |
1864 |
| # of Unigene |
1386;
Of them, 616 singletons and 770 TCs.
|
| Download (.zip) |
link |
| Release Date |
Oct 26, 2011 |
| Contact |
Haiping Xin Key Laboratory of the Ministry of Education for Plant Developmental Biology College of Life Science, Wuhan University Box3012, Hubei, 430072 China Tel: 862768756170 Fax: 862768756010 Email: xinhaiping215@hotmail.com Seq primer: pTripEX2 5' sequencing primer. |
| Citation |
Expressed sequence-tag analysis of tobacco sperm cells reveals a unique transcriptional profile and selective persistence of paternal transcripts after fertilization
Xin,H.P., Peng,X.B., Ning,J., Yan,T.T., Ma,L.G. and Sun,M.X.
Sex. Plant Reprod. 24 (1), 37-46 (2011)
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