| Detail of the metabolite profile | |
|---|---|
| Profile Acc. | Hops_GC-MS-Polar_3 |
| Analysis Method | Polar gas chromatography mass spectroscopy (GC-MS) |
| Description for analysis method | A portion of 1 mL aqueous extract was taken out and dried with speed vacuum for polar metabolite analysis. Dried polar extracts were methoximated in pyridine with 40 uL of 15.0 mg/mL methoxyamine-HCl, vetoxed thoughly and sonicated for 15 min at room temperature, followed by incubation at 50 ??C until the residue was resuspended (around 2 h). Metabolites were then derivatized with 30 uL of MSTFA+1% TMCS for 1 h at 50 ??C. The sample was subsequently transferred to a 200 uL glass insert and analysed by analysed using an Agilent 6890 GC coupled to a 5973 MSD scanning from m/z 50-650. Samples were injected at a 1:15 split ratio, and the inlet and transfer line were held at 280 ??C. Separation was achieved with a temperature programme of 80 ??C for 2 min, then ramped at 5 ??C/min to 315 ??C and held for 12 min, a 60 m DB-5MS column (J&W Scientific, 0.25 mm ID, 0.25 lm film thickness) and a constant flow of 1.0 ml/min. |
| Species / Cultivar / Tissue | Humulus lupulus / / Trichome |
| Description for biological material | Female cones were mashed in liquid nitrogen and glandular trichomes were separated from cones. The trichomes were shifted into a glass vial through a fine metal mesh. The samples were lyophilized prior to analysis. |
| Replicate No. | 3 |
| Reference | Don Sik Yang, Lloyd W. Sumner. Metabolic Profiling of trichomes from Potato Leafhopper susceptible and resistant alfalfa lines. (unpublished) |
| Contact | Lloyd Sumner Plant Biology Division The Samuel Roberts NOBLE Foundation, Inc. Ardmore, OKLAHOMA, U.S.A - 73401. 1-580-224-6710 lwsumner@noble.org |
| Download | raw .CDF file .ELU file .FIN file |
| Peaks list | Link |