| Detail of the metabolite profile | |
|---|---|
| Profile Acc. | Average_of_Hops_GC-MS-Nonpolar-Hydrolysis |
| Analysis Method | Hydrolysis non-polar gas chromatography mass spectroscopy (GC-MS) |
| Description for analysis method | A portion of 0.5 mL organic extract was dried under N2 and hydrolysed by adding 0.5 ml 1.25 M HCl in MeOH and incubating for 4 h at 50 ??C. Following hydrolysis, HCl and solvent were evaporated under nitrogen. The sample was resuspended in 30 uL pyridine and derivatized through the addition of 20 uL of a commercial derivatization solution containing MSTFA+1%TMCS (Pierce Biotechnology, Rockford, IL, USA). The sample was incubated for 1 h at 50 ??C to allow though derivatization. The sample was equilibrated to room temperature, transferred to a 200 uL glass insert, and analysed using an Agilent 6890 GC coupled to a 5973 MSD scanning from m/z 50-650. Samples were injected at a 1:1 split ratio, and the inlet and transfer line were held at 280 ?C. Separation was achieved with a temperature programme of 80 ??C for 2 min, then ramped at 5 ??C/min to 315 ??C and held for 12 min, a 60 m DB-5MS column (J&W Scientific, 0.25 mm ID, 0.25 lm film thickness) and a constant flow of 1.0 ml/min. |
| Species / Cultivar / Tissue | Humulus lupulus / / Trichome |
| Description for biological material | Female cones were mashed in liquid nitrogen and glandular trichomes were separated from cones. The trichomes were shifted into a glass vial through a fine metal mesh. The samples were lyophilized prior to analysis. |
| Replicate No. | 0 |
| Reference | Don Sik Yang, Lloyd W. Sumner. Metabolic Profiling of trichomes from Potato Leafhopper susceptible and resistant alfalfa lines. (unpublished) |
| Contact | Lloyd Sumner Plant Biology Division The Samuel Roberts NOBLE Foundation, Inc. Ardmore, OKLAHOMA, U.S.A - 73401. 1-580-224-6710 lwsumner@noble.org |
| Download | N/A |
| Peaks list | Link |