| Source Name |
Mean of Trichome Stem from Resistance Line |
| Species |
Medicago sativa potato leaf hopper resistance line, Forage Genetics International |
| Organism |
trichome and stem |
| Development Stage |
adult |
| Chip |
AffyMedicago |
| Description |
mean of Stem-Trichome-R1 and Stem-Trichome-R2.
Trichomes were collected from 20 alfalfa plants and pooled together as one replicate for both metabolome and transcriptome experiments. After the first replicate samples were collected, the plants were cut back and grow for 2 weeks. Trichomes collected from these plants were the second replicate samples. And the 3rd replicates samples were collected from the cutting back of the 2nd replicate plants. The semi-quantification will be based on the amount of stems used to collect trichome. |
| Contact |
Yuhong Tang
Microarray Facility, Plant Biology Division
The Samuel Roberts NOBLE Foundation, Inc.
Ardmore, OKLAHOMA, U.S.A - 73401.
1-580-224-6645
ytang@noble.org |
| Normalize Approach |
For each Affymetrix array hybridized, the resulting .cel file was exported from GeneChip Operating Software Version 1.4 (Affymetrix) and imported into Robust Multiarray Average (RMA; Irizarry et al., 2003) for global normalization. Presence/absence call for each probe set was obtained using dCHIP (Li and Wong, 2001). |
| Retrieve Raw Data |
link
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| Download normalized result |
link |
