| Detail of the metabolite profile | |
|---|---|
| Profile Acc. | Average_of_Hops_UPLC |
| Analysis Method | UPLC-qTOF-MS |
| Description for analysis method | Trichomes were first extracted with 1.0 mL 80% MeOH (containing 18µg/mL of umbelliferone as internal standard) in H2O. Agitation for 2 hr and centrifuge for 30 min at 2900g. 0.5 mL of the resulted extract was analyzed with UPLC/MS for secondary metabolites (UPLC/MS). A portion of 5 ?L was injected onto a reverse column (ACQUITY UPLC™ BEH C18 1.7µm, 2.1 mm x 150mm), which was maintained at 60 °C and components were eluted using a linear gradient from 95% to 30% A (eluent A, 0.1% aq. HOAc) over 30 min and at a flow rate 0.56 mL/min. The complimentary eluent B was acetonitrile. TOF-MS spectra were acquired under the following conditions: spectral acquisition rate: 3.13 per second; detector voltage: 2600 (v); threshold: 2037; ESI: -4500 v; desolvation temperature: 300 oC; nebulizer pressure: 350 kPa; interface: 100 oC. Accurate mass was achieved within 20 ppm. The chromatograms obtained from UPLC- qTOF-MS analysis were deconvoluted and aligned within mass and retention time windows prior to normalization using the Waters MarkerLynx 4.1 software (Waters) to generate a matrix of m/z and retention pairs with associated intensities resulting in a metabolite marker list. The data of mass peak intensities were collected using the following parameters: mass tolerance was set at 0.05 Da, noise elimination level at 5.00, minimum peak intensity at 40, retention time window at 0.2. |
| Species / Cultivar / Tissue | Humulus lupulus / / Trichome |
| Description for biological material | Female cones were mashed in liquid nitrogen and glandular trichomes were separated from cones. The trichomes were shifted into a glass vial through a fine metal mesh. |
| Replicate No. | 0 |
| Reference | Don Sik Yang, Lloyd W. Sumner. Metabolic Profiling of trichomes from Potato Leafhopper susceptible and resistant alfalfa lines. (unpublished) |
| Contact | Lloyd Sumner Plant Biology Division The Samuel Roberts NOBLE Foundation, Inc. Ardmore, OKLAHOMA, U.S.A - 73401. 1-580-224-6710 lwsumner@noble.org |
| Download | N/A |
| Peaks list | Link |