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Distribution of actin gene isoforms in the Arabidopsis leaf measured in microsamples from intact individual cells. Laval V, Koroleva OA, Murphy E, Lu C, Milner JJ, Hooks MA, Tomos AD. Planta. 2002 Jun;215(2):287-92. ...... (SC)-RT-PCR was used to amplify the actin transcripts using specific primer pairs for ACT1, 2, 3, 4, 7, 8, 11 and 12. Only ACT2 and ACT8 were found in epidermal and in mesophyll cells. In individual trichomes, in addition to ACT2 and ACT8, ACT7 and ACT11 transcripts were detectable. By employing the already well-characterized actin system we demonstrate the practicality and power of SC-RT-PCR as a technique for analyzing gene expression at the ultimate level of resolution, the ...... |